Crystal structure of human seminal diferric lactoferrin at 3.4 Å resolution
نویسندگان
چکیده
Janesh Kumar, Wolfgang Weber, Sabine Münchau, Savita Yadav, S Bhaskar Singh, K Saravanan, M Paramasivam, Sujata Sharma, Punit Kaur, A Bhushan, A Srinivasan, Christian Betzel and T P Singh* Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India Universitätsklinikum Hamburg-Eppendorf, IMBM, Martinistrasse 52, D-20246 Hamburg, Germany Institute of Medical Biochemistry and Molecular Biology, C/o DESY, Geb 22a Notkestrasse 85, 22603, Hamburg, Germany
منابع مشابه
Three-dimensional structure of a new form of mare lactoferrin in 70% PEG 400 at 3.8 A resolution.
Three-dimensional (3D) structure of a new form of diferric mare lactoferrin has been determined at 3.8 A resolution. The protein was crystallized in a space group P2(1)2(1)2(1) with a = 80.1 A, b = 103.7 A, c = 112.2 A with a solvent content of 57%. The structure was solved by molecular replacement method using the model of native mare lactoferrin. The structure has been refined using X-PLOR to...
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Pyrroloquinoline quinone (PQQ) is an important redox-active cofactor for many bacterial dehydrogenases. It’s biosynthetic pathway involves six or seven genes, one of which is pqqB. Former studies indicated that the protein encoded by pqqB, namely PqqB, functions as a PQQ transporter. Here we report the crystal structure of PqqB from Pseudomonas putida at 2.2 Å resolution together with functiona...
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Lactoferrin is a 703-amino acid glycoprotein originally isolated from milk. Plasma lactoferrin is predominantly neutrophil derived but indications are that it may also be produced by other cells. Lactoferrin in body fluids is found in the iron-free form, the monoferric form and in the diferric form. Three isoforms of lactoferrin have been isolated, ie two with RNase activity (lactoferrin-beta a...
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The Tp34 (TP0971) membrane lipoprotein of Treponema pallidum, an obligate human pathogen and the agent of syphilis, was previously reported to have lactoferrin binding properties. Given the non-cultivatable nature of T. pallidum, a structure-to-function approach was pursued to clarify further potential relationships between the Tp34 structural and biochemical properties and its propensity to bi...
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A sandwich e.l.i.s.a. method was developed to examine the distribution of lactoferrin in mouse reproductive tract. The lactoferrin concentration was found to be much higher in oviduct, uterus, vagina, vas deferens or epididymis than in serum, but the concentration in ovary, testis, seminal vesicle, prostate or coagulating gland was comparable with that in serum. The existence of lactoferrin in ...
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